Naba A, Clauser K.R,and Hynes R.O. Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis. J. Vis. Exp, 2015, (101), e53057, doi:10.3791/53057
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Naba A, Clauser K.R, Ding H, Whittaker C.A, Carr S.A, and Hynes R.O. The Extracellular Matrix: Tools and Insights for the « Omics » Era. Matrix Biology, 2015, pii: S0945-053X(15)00121-3
Liens vers l’article (open access): http://www.sciencedirect.com/science/article/pii/S0945053X15001213
Highlights:
• The matrisome is defined as the ensemble of 1000 + genes encoding ECM and ECM-associated proteins.
• Bioinformatic and experimental approaches to study the ECM/matrisome are discussed.
• We introduce a novel website and database MatrisomeDB to centralize resources on the matrisome.
• We present a draft of an ECM atlas compiling proteomics data on the ECM of 14 different tissues and tumors.
• “Omics” data provide novel insights into ECM functions in development, homeostasis and disease.